Table 1.
Sequence of forward and reverse primers for real-time PCR.
Fig 1.
Total cholesterol, cholesteryl esters and free cholesterol.
Plasma concentration of total cholesterol (Fig 1a), cholesteryl esters (Fig 1b) and free cholesterol (Fig 1c) in week 1 p.p., and before (week 0) and after (week 3) feed restriction in mid-lactation. Changes over time within the groups are marked with superscripts (A to C for the control group (CON); a to c for the feed-restricted group (RES); P < 0.05). Differences between control (CON) and feed-restricted (RES) group in week 3 are indicated by * (P < 0.05). Data are presented as means ± standard error of the mean.
Fig 2.
VLDL-cholesterol, LDL-cholesterol and HDL-cholesterol.
Plasma concentration of VLDL-cholesterol (Fig 2a), LDL-cholesterol (Fig 2b) and HDL-cholesterol (Fig 2c) in week 1 p.p., and before (week 0) and after (week 3) feed restriction in mid-lactation. Changes over time within the groups are marked with superscripts (A to C for the control group (CON); a to c for the feed-restricted group (RES); P < 0.05). Differences between control (CON) and feed-restricted (RES) group in week 3 are indicated by * (P < 0.05). Data are presented as means ± standard error of the mean.
Fig 3.
Plasma concentration of phospholipids (Fig 3a) and triacylgycerol (Fig 3b) in week 1 p.p., and before (week 0) and after (week 3) feed restriction in mid-lactation. Changes over time within the groups are marked with superscripts (A to C for the control group (CON); a to c for the feed-restricted group (RES); P < 0.05). Differences between control (CON) and feed-restricted (RES) group in week 3 are indicated by * (P < 0.05). Data are presented as means ± standard error of the mean.
Fig 4.
Plasma activity of lecithin:cholesterol acyltranserase (LCAT; Fig 4a) and phospholipid transfer protein (PLPT; Fig 4b) in week 1 p.p., and before (week 0) and after (week 3) feed restriction in mid-lactation. Changes over time within the groups are marked with superscripts (A and B for the control group (CON); a and b for the feed-restricted group (RES); P < 0.05). Data are presented as means ± standard error of the mean.
Fig 5.
Cholesterol concentration (mmol/l) in milk (Fig 5a), cholesterol mass (grams) in milk (Fig 5b), cholesterol concentration in milk fat (mg cholesterol per 100 g fat; Fig 5c) and fat mass (grams) in milk (Fig 5d) in week 1 p.p., and before (week 0) and after (week 3) feed restriction in mid-lactation. Changes over time within the groups are marked with superscripts (A and B for the control group (CON); a and b for the feed-restricted group (RES); P < 0.05). Data are presented as means ± standard error of the mean.
Table 2.
Relative mRNA abundances (delta CT, log2) of genes analyzed in liver biopsies in week 1 p.p., and before (week 0) and after (week 3) feed restriction in mid-lactation.