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Table 1.

Species composition of the different DNA pools used in PCR analyses.

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Table 1 Expand

Table 2.

Primer pairs used for DNA amplification [26, 27].

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Table 2 Expand

Table 3.

Libraries prepared and sequenced with Ion Torrent PGM including amplicons obtained from different DNA mixtures.

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Table 3 Expand

Fig 1.

Number of reads mapped by the three algorithms (aln, SW and mem) for each species.

The products obtained from the three primer pairs after the filtering step of sequence data obtained from library 1 were considered together.

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Fig 1 Expand

Fig 2.

Percentage of reads obtained for the different primer pairs from library 2.

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Fig 2 Expand

Fig 3.

Percentage of reads obtained from libraries 3A and 3B for the different species and primer pairs combinations.

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Fig 3 Expand

Table 4.

Pearson’s correlation between the number of mapped reads obtained from libraries 3A and 3B using different primer pairs.

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Table 4 Expand

Table 5.

Number of mapped reads for each primer pair and species combinations obtained from libraries 4 and 5 in which pig and horse amplicons were obtained from DNA mixtures in which pig and horse DNA was included at 1:10 and 1:50 ratios, respectively.

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Table 5 Expand