Fig 1.
Chemical structure of cordycepin.
Fig 2.
Chemical structure of N6-(2-hydroxyethyl)-adenosine.
Fig 3.
Effects of cordycepin on HSA by UV spectra.
A: The concentration of HSA was 0.5 μM while the cordycepin concentration corresponding to 0, 10, 20, 30, 40 and 50 μM from (1 to 6). B: 1, 0.5 μM HSA + 50 μM cordycepin; 2, 50 μM cordycepin; 3, 0.5 μM HSA.
Fig 4.
Effects of HEA on HSA by UV spectra.
A: The concentration of HSA was 0.5 μM while the HEA concentration corresponded to 0, 10, 20, 30, 40 and 50 μM from (1 to 6). B: 1, 0.5 μM HSA + 50 μM HEA; 2, 50 μM HEA; 3, 0.5 μM HSA.
Fig 5.
Effect of drug on fluorescence spectra of HSA.
A: Effect of cordycepin on fluorescence spectra of HSA; the concentration of HSA was 1 μM while the cordycepin concentration corresponded to 0, 10, 20, 30, 40 and 50 μM (from 1 to 6). B: Effect of HEA on fluorescence spectra of HSA. The concentration of HSA was 1 μM while the HEA concentration corresponding to 0, 10, 20, 30, 40 and 50 μM (from 1 to 6).
Fig 6.
A: Stern–Volmer plot for cordycepin–HSA at different temperatures; B: Stern–Volmer plot for HEA–HSA at different temperatures.
Table 1.
Stern-Volmer equations and quenching constants for fluorescence quenching of HSA by cordycepin and HEA at different temperatures.
Fig 7.
Plot of log [(F0-F)/F] vs log [Q] at different temperatures of cordycepin and has (A); Plot of log [(F0-F)/F] vs log [Q] at different temperatures of HEA and has (B).
Table 2.
K values of HSA at different temperatures.
Fig 8.
ΔH, ΔG, and ΔS of the reaction between cordycepin and has (A).
ΔH, ΔG, and ΔS of the reaction between HEA and has (B).
Table 3.
ΔH, ΔG, and ΔS of the interaction between cordycepin and HSA, HEA and HSA at different temperatures.
Fig 9.
Stern-Volmer equations and quenching constants for fluorescence quenching of HSA by cordycepin and HEA at different temperatures.
C(cordycepin) = C (HSA) = C (HEA) = 1 μM.