Fig 1.
Map of study area with environmental DNA sample collection locations (N = 21) and Burmese python occupancy.
Locations with present and absent environmental DNA detections are indicated with black and white dots, respectively. Some sample locations overlap due to the scale. BDB, Bird Drive Basin; DE, Deering Estates; ENP, Everglades National Park; HLWM, Holey Lands Wildlife Management Area; STA, Stormwater Treatment Area 5. Southwest Florida samples (SWFL) include three radiotagged Burmese pythons: ELV, Elvis; NOS, Noosa; and SWP, Sweet Pea.
Table 1.
Environmental DNA quantitative PCR (qPCR) primer and probe sequences, annealing temperature (TA), and product size in base-pairs for five giant constrictor invasive species.
Table 2.
Relative quantification (relative standard curve) values for five giant constrictor species using genomic DNA (gDNA) and absolute quantification (standard curve) values for Python molurus bivittatus using a synthetic gene.
Fig 2.
Estimated concentration of Burmese python environmental DNA (mean and 95% confidence interval) per sample.
a. Higher concentration regions, y-axis scaled from 0 to 10 molecules/mL. b. Lower concentration regions, y-axis scaled from 0.0 to 1.4 molecules/mL. From each region, one to three locations were targeted, with triplicate field samples collected at each location. BDB, Bird Drive Basin; DE, Deering Estates; ENP, Everglades National Park; HLWM, Holey Lands Wildlife Management Area; SWP, Sweet Pea; NOS, Noosa; ELV, Elvis; STA, Stormwater Treatment Area 5.
Table 3.
Regional Bayesian estimates of occurrence (ψ) and detection (p) probabilities of Python molurus bivittatus environmental DNA.
Fig 3.
The effect of sample size on estimates of the cumulative probability of occurrence of Burmese python environmental DNA (θ*) associated with samples taken from the four field locations analyzed by the three-level occupancy model.
BDB, Bird Drive Basin; DE, Deering Estates; ENP, Everglades National Park; STA, Stormwater Treatment Area 5.