Fig 1.
Fruits of P. avium L. ‘13–33’ and ‘Tieton’ used in deep sequencing.
(A) ‘13–33’ fruit at 20 DAF (stage 1). (B) ‘13–33’ fruit at 35 DAF (stage 2). (C) ‘13–33’ fruit at 45 DAF (stage 3). (D) ‘13–33’ fruit at 55 DAF (stage 4). (E) ‘Tieton’ fruit at 20 DAF (stage 1). (F) ‘Tieton’ fruit at 35 DAF (stage 2). (G) ‘Tieton’ fruit at 45 DAF (stage 3). (H) ‘Tieton’ fruit at 55 DAF (stage 4).
Table 1.
Summary of the sequencing and de novo assembly.
Table 2.
Summary of the annotations for the assembled sweet cherry unigenes in public databases.
Fig 2.
E-value and similarity distribution of sweet cherry fruit tissue transcriptome unigenes with annotation to the Nr database.
(A) E-value distribution of annotated unigenes; (B) Similarity distribution of annotated unigenes.
Fig 3.
GO classification of unigenes of P. avium L fruit.
The results are summarized in three main GO categories: Biological process, Cellular Component and Molecular Function.
Fig 4.
Histogram of KOG classification.
The 8,645 unigenes were grouped into 25 KOG categories.
Table 3.
Statistics of DGE library sequencing and read mapping.
Fig 5.
Unigenes differentially expressed between different stages of fruit development and between cultivars at the same stage.
Upregulated (red) and downregulated (green) unigenes were quantified. The results of 14 comparisons between each two samples are shown.
Fig 6.
Comparison of anthocyanin content in different ripening stages of the red sweet cherry cultivar ‘Tieton’ and the yellow sweet cherry cultivar ‘13–33’.
Fig 7.
Unigenes involved in the anthocyanin biosynthesis of sweet cherry.
The numbers in parentheses indicate the number of unigenes for each gene in the transcriptome library.
Table 4.
Expression profiles of anthocyanin biosynthesis genes in sweet cherry fruit.
Fig 8.
Expression analysis of 18 candidate DEGs related to anthocyanin biosynthesis in sweet cherry by qRT-PCR.
The Y-axis represents the relative expression, and the X-axis depicts the fruit ripening stages. The standard error of the mean for three biological replicates (nested with three technical replicates) is represented by the error bars. Different letters on each symbol indicate statistically significant differences (P < 0.05) between two values according to ANOVA and Duncan’s new multiple range tests.