Fig 1.
Phylogenetic tree of IFIT5 amino acid sequences generated with the neighbor-joining tree method.
Numbers at each branch indicate the percent a node was supported in 1,000 bootstrap replicates.
Fig 2.
The predicted conserved domains predicted from the amino acid sequence of duIFIT5 and human IFIT5.
Both duIFIT5and human IFIT5 have eight TPR motifs and multi-domains TPR_11 and TPR_12.
Fig 3.
Subcellular localization of duIFIT5 in DF1 cells.
EGFP-C1-duIFIT5 or an EGFP-C1 control plasmid was transiently transfected into DF1 cells, shown in A and B, respectively. DuIFIT5 was mostly in the cytoplasmic, but was also detected in the nucleus.
Fig 4.
Relative expression levels of duIFIT5 in the following tissues: heart, liver, spleen, lung, kidney, cerebrum, cerebellum, large intestine, small intestine, glandular stomach, muscular stomach, and muscle.
The expression of duIFIT5 was normalized to GAPDH. Different letter showed significant difference (p < 0.05).
Fig 5.
Relative expression of duIFIT5 in liver (A) and spleen (B) after DHV-1 injection.
qRT-PCR was used to determine the relative expression of duIFIT5 in liver and spleen tissues at 0, 4, 8, 12, 24, 36, 48, 72 and 96 h after infection with DHV-1. The expression of duIFIT5 was normalized to GAPDH. Different letter showed significant difference (p < 0.05).
Fig 6.
Relative expression of duIFIT5 in liver (A) and spleen (B) after poly (I:C) injection.
qRT-PCR was used to determine the relative expression of duIFIT5 in liver and spleen tissues at 0, 4, 8, 12, 24, 36, 48, 72 and 96 h after infection with poly (I:C). The expression of duIFIT5 was normalized to GAPDH. Different letter showed significant difference (p < 0.05).