Fig 1.
Scion/ rootstock fresh weight and hardness in watermelon (Citrullus vulgaris Schrad.) ‘Sambok Honey’ and ‘Speed’ as the scion and bottle gourd (Lagenaria siceraria Stanld.) ‘RS Dongjanggun’ as rootstock seedlings grown under different photon flux densities (25, 50, 75 and 100 μmol m−2 s−1 PPFD).
(A-C) scion/rootstock fresh weight of ‘Sambok-Honey’ (B-D) scion/ rootstock fresh weight of ‘Speed’ (E) hardness of ‘Sambok-Honey’ (F) hardness of ‘Speed’.
Fig 2.
2-DE reference maps of vascular connections (connected portions) in watermelon (Citrullus vulgaris Schrad.) ‘Sambok Honey’ as the scion and bottle gourd (Lagenaria siceraria Stanld.) ‘RS Dongjanggun’ as rootstock seedlings grown under different photon flux densities (25, 50, 75 and 100 μmol m−2 s−1 PPFD).
Proteins from vascular connections (70 μg) were elctrofocused on a pH 4–7 IPG strip (11 cm), separated onto 12.5% (w/v) SDS-PAGE. The gels were silver stained and visualized as described in experimental section. Protein spots marked by arrows were identified by MALDI-TOF/TOF-MS as described in experimental sections.
Fig 3.
2-DE reference maps of vascular connections (connected portions) in watermelon (Citrullus vulgaris Schrad.) ‘Speed’ as the scion and bottle gourd (Lagenaria siceraria Stanld.) ‘RS Dongjanggun’ as rootstock seedlings grown under different photon flux densities (25, 50, 75 and 100 μmol m−2 s−1 PPFD).
Proteins from vascular connections (70 μg) were elctrofocused on a pH 4–7 IPG strip (11 cm), separated onto 12.5% (w/v) SDS-PAGE. The gels were silver stained and visualized as described in experimental section. Protein spots marked by arrows were identified by MALDI-TOF/TOF-MS as described in experimental sections.
Table 1.
Proteins identified by MALDI-TOF/TOF-MS in watermelon ‘Sambok Honey’ as the scion and bottle gourd ‘RS Dongjanggun’ as rootstock.
Fig 4.
Functional classification of identified proteins from vascular connections (connected portions) in watermelon (Citrullus vulgaris Schrad.) ‘Sambok Honey’ and ‘Speed’ as the scion and bottle gourd (Lagenaria siceraria Stanld.) ‘RS Dongjanggun’ as rootstock seedlings grown under different photon flux densities (25, 50, 75 and 100 μmol m−2 s−1 PPFD).
(A) ‘Sambok honey’ (B) ‘Speed’ as the scion and bottle gourd (Lagenaria siceraria Stanld.) ‘RS Dongjanggun’ as rootstock seedlings grown under different photon flux densities (25, 50, 75 and 100 μmol m−2 s−1 PPFD). The proteins identified were classified based on their putative biological functions.
Table 2.
Proteins identified by MALDI-TOF/TOF-MS in watermelon ‘Speed’ as the scion and bottle gourd ‘RS Dongjanggun’ as rootstock.
Fig 5.
Comparative analysis of vascular connection proteome.
A graphical representation of the number of differentially accumulated proteins in watermelon (Citrullus vulgaris Schrad.) ‘Sambok Honey’ and ‘Speed’ as the scion and bottle gourd (Lagenaria siceraria Stanld.) ‘RS Dongjanggun’ as rootstock seedlings grown under different photon flux densities (25, 50, 75 and 100 μmol m−2 s−1 PPFD).
(A) ‘Sambok honey’ (B) ‘Speed’ as the scion and bottle gourd (Lagenaria siceraria Stanld.) ‘RS Dongjanggun’ as rootstock. Numbers correspond to the protein spots present in 2DE patterns under 25, 50, 75 and 100 μmol m−2 s−1. The overlapped portions with ‘X’ denote absence of proteins. Upward and downward arrows denote increased or decreased protein expression under combined treatments.
Fig 6.
Visualization of water uptake and transport in watermelon (Citrullus vulgaris Schrad.) ‘Sambok Honey’ and ‘Speed’ as the scion and bottle gourd (Lagenaria siceraria Stanld.) ‘RS Dongjanggun’ as rootstock seedlings grown under different photon flux densities (25, 50, 75 and 100 μmol m−2 s−1 PPFD).
The rootstock were submerged in absorbable flower dye blue for 10–20 min and both rootstock and scion were cut into transverse and longitudinal cross section and were observed under light microscope. The arrows in vascular tissues represent the vascular transport activity. (A-B) represents longitudinal section without flower dye blue; (C-D) represents longitudinal section with flower dye blue; (E-F) represents transverse section without flower dye; (G-H) represents transverse section with flower dye blue in rootstock and scion.