Fig 1.
Geographical distribution of the sampling areas in Tenerife.
1, El Pris; 2, Pico del Inglés; 3, La Esperanza; 4, La Laguna; 5, Pedro Álvarez.
Fig 2.
Mollusc species included in the study.
Theba pisana (a), Cornu aspersum (b), Plutonia lamarckii (c).
Fig 3.
Detection of Angiostrongylus cantonensis by LAMP reaction.
tube 1, DNA isolated from Plutonia lamarckii tissue; tube 2, DNA obtained from A. cantonensis adult worms; tube 3, negative control.
Fig 4.
Light microscopy of third stage larvae (L3) morphologically compatible with Angiostrongylus cantonensis, obtained from Theba pisana.
General view of third-stage larvae with one sheath (S). A characteristic “T”-shaped structure (TS) is apparent at the anterior end of the sheath that surrounds the L3 larva (a). Posterior end of L3 larva with anus (A) at subterminal position. Anus cuticle (AC) is completely molted and can be seen on the sheath (b). Anterior end of L3 larva with esophagus bulbus (EB), excretory pore (EP) and nervous ring (NR) (c).
Table 1.
Percentage prevalence of Angiostrongylus cantonensis larvae in molluscs and numbers of molluscs testing positive in samples from Tenerife, based on morphological (P) and molecular identification (P*).