Fig 1.
Structures of the paulomycins, paulomenols and two of their analogs decorated at the paulic acid moiety, paldimycin A and U-77802.
Fig 2.
Genetic organization of the pau gene cluster from S. paulus NRRL 8115.
Proposed functions for individual ORFs are coded with various patterns and summarized in Table 1.
Table 1.
Homologous proteins of ORFs in the paulomycin biosynthetic gene clusters.
Fig 3.
HPLC traces of selected S. paulus mutants and recombinant strains.
CIM3001, the pau11 inactivated mutant; CIM3003, complemented strain of CIM3001; CIM3002, the pau18 inactivated mutant; CIM3004, complemented strain of CIM3002; CIM3005, the pau13 inactivated mutant; CIM3006, complemented strain of CIM3005; CIM3007, S. paulus recombinant strain overexpressing the pau13 gene. Paulomycin A (□); Paulomycin B (○); Paulomenol A (⃟); Paulomenol B (Δ).
Fig 4.
A proposed convergent model of paulomycin biosynthesis.