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Fig 1.

Treatment process for the chosen municipal wastewater treatment plant.

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Table 1.

The primers of four ERY and four TC resistance genes used in the study.

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Fig 2.

Heterotrophic bacteria resistant to nine antibiotics after different doses of chlorination.

VAN, CEP, SD, ERY, RIF, GEN, TC, CIP, CHL and ERY&TC represent. vancomycin-, cephalexin-, sulfadiazine-, erythromycin-, rifampicin-, gentamicin-, tetracycline-, ciprofloxacin-, chloramphenicol and erythromycin & tetracycline- resistant bacteria, respectively. Error bars indicate standard deviation for replicates from a single sample.

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Fig 3.

Abundance of four ERY resistance genes (a) and four TC resistance genes (b) in samples treated by chlorination under a series of doses (mg Cl2 min/L).

Error bars indicate standard deviation for replicates from a single sample.

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Fig 4.

Ratio curves for ERY- and TC- resistant bacteria and genes under a series of doses (mg Cl2 min/L).

Ratio = Ni/N0, where N0: Initial concentrations of ERY- or TC- resistant bacteria or genes in samples before chlorination; Ni: Concentrations of ERY- or TC- resistant bacteria or genes in samples treated by chlorination at a certain dose.

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