Table 1.
The partial information of plasmid-assembled.
Table 2.
The results of ncRNA prediction.
Table 3.
The comparative analysis of insecticidal activity substances in B.thuringiensis 4.0718 in genomics and proteomics.
Fig 1.
Protein identified of phases T1, T2 and T3 by mass spectrometry compared with the results of genome annotation.
The part of circle outer represents the proteins that can be detected by LC-MS/MS but can not be searched from the results of the genome annotation.
Table 4.
The results of the Mass spectrometry-based proteomic data compare with genome annotation data.
Table 5.
The statistics of not found proteins for the result of genome annotation with comparison the result of the LC-MS/MS searched.
Fig 2.
Analysis of start codon and initiator methionine for acetate CoA-transferase, alpha subunit.
Red arrow, initiator methionine, as identified by Prodigal.v2_60 software. Green arrow, initiator methionine of the protein identified by LC-MS/MS through received uniprot blast. These initiator methionines were determined from B. thuringiensis subsp. konkukian (strain 97–27) and B. thuringiensis serovar kurstaki strain T03a001, respectively.
Fig 3.
Complete circuit diagram of key regulatory genes in sporulation.
Spo0A is a key protein that directs the transcriptional regulation of downstream gene, including asymmetric division and the expression of SigF and SigE, which are special transcription factors of pre-spore and mother cell, respectively. The two transcription factors decide the regulation of the development of spore. Red line, inhibition; Orange line, activation; Green, translation.
Table 6.
The differential expression analysis of sporulation and regulation proteins related to the spore formation in genomic and proteomic.
Fig 4.
Analysis of insecticidal Cry protein regulation network.
The figure exhibits that the insecticidal Cry protein interacts with beta-lactamase type II. Beta-lactamase type II is also regulated by LysR family transcriptional regulator.