Table 1.
ASIV improves the cardiac hemodynamics in rats.
Fig 1.
Effects of ASIV on index of cardiac hypertrophy and mRNA expression of ANP and BNP in hypertrophic heart.
A: Left ventricular tissue section stained with H&E. B: ratios of heart weight/body weight (HW/BW) and left ventricular weight/body weight (LVW/BW). C: Representative of H&E staining of heart tissue; D: Statistical data of myocyte surface area shown in H&E staining measured and analyzed with LAS Software (V4.3) (Leica, Germany). E: mRNA expression of ANP. F: mRNA expression of BNP. Data are presented as mean ±S.D. n = 8 for A, B, C and D; n = 4 for E and F. *: P<0.05 is considered statistical significance.
Fig 2.
Effect of ASIV on Iso-induced NRVM cells.
A: The cell morphology was visualized by immunofluorescence analysis. B: Cell surfaced area was measured and analyzed with LAS Software (V4.3) (Leica, Germany). C: mRNA expression of ANP. D: mRNA expression of BNP. E: Protein content. Data are presented as mean ±S.D. n = 4. *: P<0.05 is considered statistical significance.
Fig 3.
Effects of ASIV on Iso-induced changes in the energy biosynthesis and expression of ATP5D.
A and E: ATP/AMP ratio in vivo or in vitro. B and F: FFA level in vivo or in vitro. C and G: Representative of western blot photograph of ATP5D in vivo or in vitro. D and H: Statistical data of protein expression of ATP5D. Data are presented as mean ±S.D. n = 8 for A, B, C and D; n = 4 for E, F, G and H. *: P<0.05 is considered statistical significance.
Fig 4.
Effects of ASIV on p65 and PGC-1α protein expression measured by Western blot.
A and B: p65 expression in cytoplasm and nuclear fraction of heart tissue. C and D: p65 expression in cytoplasm and nuclear fraction of NRVMs.B: E and F: PGC-1α expression in heart tissue and NRVMs. Data are presented as mean ±S.D. n = 4. *: P<0.05 is considered statistical significance.
Fig 5.
Effect of p65 knockdown on surface area and PGC-1α protein expression.
A: representative cell morphology (upper panel); Statistical data of cell surface area (lower panel). B: PGC-1α protein expression in NRVMs.