Table 1.
Primer sequences for RT-PCR.
Fig 1.
Log-Log Scatter Plot of normal and mastitis mammary tissues.
Red replaces higher expressed genes in mastitis tissue. Green replaces the higher expressed genes in normal tissue.
Fig 2.
Analysis of 79 differently expressed genes of TLR4/ NF-kB signaling pathway in normal and mastitis mammary tissues (P<0.01).
The microarray heat map demonstrates the log2 ratio of gene signal difference between normal and mastitis tissues. Up-regulated genes are shown in red, while down-regulated genes are shown in green. The left column on the figure represents the level of expression in normal mammary tissue, while the right column represents the level of corresponding gene expression in mastitis mammary tissue.
Table 2.
Differentially expressed genes in mastitis mammary tissues with respect to normal mammary tissues.
Fig 3.
Biologial process analyzed of genes that changed two-fold ≥ 2 folds in normal (A) and mastitis mammary tissue (B) by Gene ontology analysis (P values < 0.01).
Fig 4.
Biologial process analyzed of genes that changed two-fold ≥ 2 folds in normal (A) and mastitis mammary tissue (B) by Gene KEGG analysis (P values < 0.01).
Fig 5.
Microarray results confirmed by RT-PCR.
A. RT-PCR results of genes selected. B. Comparison of RT-PCR findings to microarray results by fold-change of ten-selected genes. N = normal tissue and m = mastitis tissue. Note: a single asterisk indicates a statistical difference (P< 0.05), and a double asterisk indicates a statistical difference (P<0.01).
Fig 6.
Microarray results confirmed by western blot.
Note: a double asterisk indicates a statistical difference (P<0.01).