Fig. 1.
Control compounds effect on biofilm formation.
S. aureus (SC01) biofilm formation was evaluated after 24 h (A) and 48 h (B) in the presence of D-amino acids previously reported as inhibitors at a concentration of 1 mM or the positive control carvacrol (2 mM). The absorbance was recorded at 595 nm following crystal violet staining. All points are significantly different from the positive control carvacrol (****p<0.0001) relative to positive control, unpaired t-test.).
Fig. 2.
Effect of increased concentration of control compounds.
S. aureus (SC01) biofilm formation was evaluated visually (A) and spectroscopically (B) after 24 h in the presence of D-tyrosine, D-tryptophan, and an equimolar mixture of D-tyrosine/D-proline/D-phenylalanine at a concentration of 5 mM.
Fig. 3.
Diverse set of D-amino acids effect on biofilm formation.
S. aureus (SC01) biofilm formation was evaluated after 24 h in the presence of specified unnatural D-amino acids at a concentration of 1 mM or the positive control carvacrol (2 mM). The absorbance was recorded at 595 nm following crystal violet staining. All points are significantly different from the positive control carvacrol (****p<0.0001 relative to positive control, unpaired t-test.).
Fig. 4.
Diverse set of D-amino acids effect on biofilm formation.
S. aureus (SC01) biofilm formation was evaluated after 48 h in the presence of specified unnatural D-amino acids at a concentration of 1 mM or the positive control carvacrol (2 mM). The absorbance was recorded at 595 nm following crystal violet staining. All points are significantly different from the positive control carvacrol (****p<0.0001 relative to positive control, unpaired t-test.).