Figure 1.
Muscle pathology in 10 month-old GM muscle of wild-type, mdx and mdx/utrn+/- mice.
Extent of total collagen staining (blue) in 10 month-old wild-type (A), mdx (B) and mdx/utrn+/- (C) GM muscle was used as a marker of fibrosis. Proportion of centrally nucleated fibers in the same tissues (D, E, and F) were measured to assess extent of regeneration. Quantification of total collagen staining (G) and proportion of centrally-nucleated myofibers (H) is represented as the mean +SD. * represents p<0.05 and ** represents p<0.01 (scale bar = 100μm).
Figure 2.
Muscle pathology in 10 month-old diaphragm muscle of wild-type, mdx and mdx/utrn+/- mice.
Extent of total collagen staining (blue) in 10 month-old wild-type (A), mdx (B) and mdx/utrn+/- (C) diaphragms was used as a marker of fibrosis. Proportion of centrally nucleated fibers in the same tissues (D, E, and F) were measured to assess extent of regeneration. Quantification of total collagen staining (G) and proportion of centrally-nucleated myofibers (H) is represented as the mean +SD. * represents p<0.05 and *** represents p<0.001 (scale bar = 100μm).
Figure 3.
Muscle pathology in 8 week-old GM muscle of wild-type, mdx, mdx/utrn+/- and dko mice.
Extent of total collagen staining (blue) in 8 week-old wild-type (A), mdx (B), mdx/utrn+/- (C) and dko (D) GM muscles was used as a marker of fibrosis. Proportion of centrally nucleated fibers in the same tissues (E, F, G, and H) were measured to assess extent of regeneration. Quantification of total collagen staining (I) and proportion of centrally-nucleated myofibers (J) is represented as the mean +SD. * represents p<0.05, ** represents p<0.01, and *** represents p<0.001 (scale bar = 100μm).
Figure 4.
Muscle pathology in 8 week-old diaphragm muscle of wild-type, mdx, mdx/utrn+/- and dko mice.
Extent of total collagen staining (blue) in 8 week-old wild-type (A), mdx (B), mdx/utrn+/- (C) and dko (D) diaphragms was used as a marker of fibrosis. Proportion of centrally nucleated fibers in the same tissues (E, F, G, and H) were measured to assess extent of regeneration. Quantification of total collagen staining (I) and proportion of centrally-nucleated myofibers (J) is represented as the mean +SD. * represents p<0.05, ** represents p<0.01, and *** represents p<0.001 (scale bar = 100μm)
Figure 5.
Comparison of histopathology of 12 week-old mdx/utrn+/- diaphragm, quadriceps, soleus, tibialis anterior and gastrocnemius muscles.
Extent of collagen staining (A,C,E,G,I) and muscle regeneration (B,D,F,H,J) in 12 week-old mdx/utrn+/- mice. Quantification of total collagen staining (K) and centrally-nucleated myofibers (CNF, L) is represented as the mean ±SD. * represents p<0.05, ** represents p<0.01, and *** represents p<0.001 (scale bar = 100μm). TA = tibialis anterior, GM = gastrocnemius.