Figure 1.
Neighbor-joining phylogenetic tree of 35 species on FTO coding sequences showing the evolutionary relationship.
The bootstrap consensus tree inferred from 1000 replicates is generated to present the evolutionary relationship among 35 species on FTO coding sequences which were retrieved from Ensembl or NCBI. Accession numbers are listed at S1 Table. Alignment was carried out by ClustalW (1581 nucleotides left) and phylogenetic tree is constructed by neighbor-joining method in MEGA6. Branches corresponding to partitions reproduced in less than 50% bootstrap replicates did no display. This tree works as the initial tree for further PAML analysis.
Table 1.
PAML analysis of FTO.
Figure 2.
Tajima’s D within the FTO locus from five populations showing signals of selection.
Sliding window analysis of Tajima’s D in the whole gene locus (∼ 1 Mb) was conducted with DnaSP version 4.50.0.3. Populations of European, African, Chinese, Japanese and Sorbs were included in the analysis. Closely related individuals were removed, i.e. trios from Chinese and Japanese populations and individuals with IBD > 0.05 from Sorbs. Higher values of Tajima’s D were observed at the intron 3.
Table 2.
Results of the DnaSP Analyses.
Table 3.
Population genetic measures on unstandardized iHS and Fst.