Fig 1.
UV-Vis spectral analysis of native (---) and 3-DG-glycated (- - - -) H3 histone.
Table 1.
Side chain modifications, formation of various intermediates and AGEs.
Fig 2.
HPLC analysis of native and 3-DG-glycated H3 histone; (A) Chromatogram of native H3, (B) & (C) standard CML and pentosidine, and (D) chromatogram of glycated-H3, respectively.
Fig 3.
Fluorescence spectral analysis of native (---) and 3-DG-glycated (- - -) H3 histone excited at, (A) 335 nm, and (B) excited at 365 nm respectively.
Fig 4.
CD spectral, thermal denaturation and FTIR profile of native (---) and 3-DG-glycated (- - - -) H3 histone; (A) Far-UV CD profiles, (B) thermal denaturation showing changes in ellipticity at 222 nm, and (C) FTIR profiles of Amide I and Amide II bands corresponding to native H3 histones and 3-DG-glycated H3 histone.