Table 1.
Bacterial stains used to determine the host range of phage PPA-ABTNL.
Table 2.
Antibiotic susceptibility of P. aeruginosa from mink.
Figure 1.
Transmission electron micrograph of phage PPA-ABTNL negatively stained with uranyl acetate.
Scale bar represents 50 nm.
Figure 2.
One-step growth curve of phage PPA-ABTNL.
PPA-ABTNL phage was co-incubated with culture of PA5-1-1 to absorb for 5 min at 37°C. The mixture was centrifuged to remove non-absorbed phage. The re-suspended pellets were incubated at 37°C and sampled at 10 min interval over a period of 2 h. Phage titer was measured. Data represent mean±SD from three independent experiments. n = 3.
Figure 3.
Antibacterial curve of phage PPA-ABTNL at different multiplication of infection in vitro.
PA5-1-1 strain was lysed by the phage PPA-ABTNL in LB medium at 37°C. OD600 development of uninfected control culture and parallel cultures infected with phage at different multiplicities of infection (MOI) was measured. n = 3.
Figure 4.
Overview of the PPA-ABTNL genome sequence analysis.
Predicted open reading frames (ORFs) are numbered. ORFs with significant homology to known genes and to the identified proteins are in dark blue. Putative genome organization. Putaitve promoters (↳), terminators () are indicated. Predicted and presumed proteins and corresponding ORF number are marked.
Table 3.
Incidence of histomorphological observations.
Figure 5.
Phage PPA-ABTNL cures mink hemorrhagic pneumonia caused by a clinical P. aeruginosa strain.
(A) Effect of atomization on the titer of phage. (B) Effect of different multiplicities of infection (MOIs) on the amount of bacteria in the lung of mink. (C) Effect of different MOIs on the amount of phage in the lung of mink. (D) Survival curves of mink infected with PA5-1-1 strain and treated with phage PPA-ABTNL. n = 5. Means with different superscripts differ significantly from each other (P<0.05).