Fig 1.
Experimental approach and and set-up for photo-attachment.
A: Chemical reaction approach for photo-attachment of biomolecules on nanowire surface. B: Simple set up for photo-attachment. The black parts in Fig. 1B represents opaque region of photomask. The yellow region represents photo-attached nanowires.
Fig 2.
Results of photo-attached spots of different diameters.
A: Array Fabrication of photo-attached spots of various diameter, yellow arrow pointing anomaly surface area resulted from nanowire fabrication process. B: Schematic diagram showing the approach to immobilize different probes at different positions separated by specially designed gaskets (black portion) and C: Piezo dispensing by Scienion AG, with permission to reproduce image.
Fig 3.
Comparison of crosslinking efficiency between chemical immobilization and photo-attachment.
Fig 4.
Photo-attachment to crosslink sense oligo on nanowire substrates.
A: Psoralen functionalized nanowire substrate, B: Diazirine functionalized nanowire substrate, C: Psoralen functionalized polished silicon substrate with the inset shows the same chip scanned at maximum power and PMT gain, D: Diazirine functionalized polished silicon substrate with the inset shows the same chip scanned at maximum power and PMT gain, E: Psoralen functionalized with passivated substrate, and F: Diazirine functionalized with passivated substrate.
Table 1.
Estimation of surface bound amine group for flat Si wafer and GLAF-MACE (Chip1 and Chip 2) surfaces.
Fig 5.
Results on nonspecific adhesion study.
A: Psoralen functionalized nanowire surface and B: Diazirine functionalized nanowire surface.
Fig 6.
Hybridization of target oligo on photo-attached substrates.
A: Psoralen functionalized substrates, B: Diazirine functionalized substrates, C: Psoralen functionalized substrate with passivation, and D: Diazirine functionalized substrate with passivation.
Fig 7.
Results of discrimination of nucleotide with single base mismatch.
A: Without formamide and B: With formamide on Psoralen functionalized surface.
Fig 8.
Probe crosslinking on nanowires with different probe structures.
A: On psoralen functionalized surface passivated with adipic acid and B: On diazirine functionalized surface passivated with adipic acid.
Fig 9.
Photo-attachment results on different surfaces.
A: On psoralen/COOH surface and B: On diazirine/COOH surface at wavelengths of 254 nm, 302 nm and 365nm with a probe concentration of 20 μM. C and D: Hybridization on nanowires crosslinked with 20 μM probes, E and F: Hybridization on nanowires crosslinked with 2 μM probes, Note that the bars indicate sense crosslinking and the lines indicate hybridization.