Fig 1.
Comparison of anti-Leishmania antibody responses in rKLO8- and rK39 ELISA using patient and control sera from three endemic regions.
Protein concentrations of 5ng/100μl rKLO8 or rK39 were tested with sera of patients and controls from Sudan (A), India (B) and France (C). Sera were tested at dilutions of 1:800. A cut off value of 0.12 was established as means + 3 SD of 30 healthy controls. VL, visceral leishmaniasis; VLS, VL suspects; HC, healthy control; MA, malaria; TP, toxoplasmosis; VL/HIV, VL and HIV, ASC, asymptomatic cases. The black horizontal lines represent cut off values. OD values of 0.6 (5 fold of cut off) and 2.15 are shown in dotted lines.
Fig 2.
Reactivity of VL sera from different endemic regions in rKLO8- and rK39 ELISA.
OD values of VL sera from Sudan (n = 50), India (n = 26) and France with negative (HIV-, n = 26) or positive (HIV+, n = 11) HIV were tested in rKLO8- or rK39 ELISA at dilutions of 1:800. Means ODs of VL sera from the different regions were compared. Dots represent values for individual sera and horizontal line represents cut-off values (0.12). Significance was determined by unpaired Student's t test at 95% confidence intervals and P-values < 0.05 indicate statistically significant differences; ns indicates no significant differences.
Table 1.
Performance of five serological tests for diagnosis of visceral leishmaniasis in Sudan, India and France.
Table 2.
Distribution of direct agglutination test (DAT) antibody titres in sera of visceral leishmaniasis patients from three endemic regions.