Figure 1.
Transmission electron microscopy of negatively stained SU10. Measured head length is 137±4.2 nm.
Table 1.
General features of the phage SU10 genome.
Figure 2.
The genome of phage SU10. The predicted CDSs are indicated by arrows, and the directions of arrows indicate direction of transcription.
28% of the predicted proteins were identified in our mass-spectrometry based proteomics. CDSs predicted to encoding structural proteins are indicated in blue, hypothetical proteins in grey, DNA replication and recombination proteins in green. Genes predicted to encode enzymes involved in nucleotide metabolism are indicated in yellow, black is showing other proteins.
Table 2.
Proteins identified by hybrid LTQ-Orbitrap Velos mass spectrometer.
Figure 3.
Ultra-thin section electron microscopy SU10. Phage empty capsids are attached to the bacterial surface receptors and their genomes are injected to the host.
Phage progenies heads are standing side by side in the bacterial cytoplasm and forming a honeycomb shape structure. Some of the heads are filled by genomes and some are in the stage of genome packaging.
Figure 4.
Scanning electron microscopy image of SU10 five minutes after adding them to the bacterial culture.
Empty phage particles can be seen adsorbed onto the surface of the host cell.
Figure 5.
Phylogenetic tree showing the relationship of the two structural proteins, the scaffold protein and the head protein, within and between the two morphotypes.
Phages SU10, NJ01, PhiEco32, GAP52, 7–11, and KSP100 belong to the C3 morphotypes (elongated capsid), and phages tf, UVF-P2, LUZ24, PaeP-p2-10, MR299-2, and PaP3 to the C1 morphotype (icosahedral capsid). The tree was inferred by using the Maximum Likelihood method based on the Whelan and Goldman model [23]. The tree with the highest log likelihood (−9785.9307) is shown. A maximum parsimony (MP) tree was used as an initial tree in the heuristic search. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analyses were conducted in MEGA6 [21].