Table 1.
The primers and probe sequences used in real-time PCR assay.
Figure 1.
Cytotoxic activity of lutein epoxide, lupeol epoxide, eugenol epoxide, lutein, lupeol and eugenol against MCF7, MDA-MB231 and MCF 10A cell lines.
Figure 2.
Time dependency effects of p53 and bcl-2 mRNA levels in human breast cancer cell line, MCF7, incubated with lutein, lupeol, eugenol, lutein epoxide, lupeol epoxide, eugenol epoxide at 1/4 of CC50 values for 6 h and 12 h incubation.
gapdh was used as an endogenous control gene. The stars indicate that the data are significantly different (p<0.05) from the untreated control.
Figure 3.
Time dependency effects of caspase 3 and bax mRNA levels in human breast cancer cell line, MCF7 and MDA-MB 231 incubated with lutein, lupeol, eugenol, lutein epoxide, lupeol epoxide, eugenol epoxide at 1/4 of CC50 values for 6 h and 12 h incubation.
gapdh was used as an endogenous control gene. The stars indicate that the data are significantly different (p<0.05) from the untreated control.
Figure 4.
Western blot analysis of MDA-MB-231 and MCF7 treated with lutein (A), lupeol (B), eugenol (C), lutein epoxide (D), lupeol epoxide (E) and eugenol epoxide (F).
Western blot analysis was performed with monoclonal antibodies to human bcl-2, p53 and bax. β-actin was used as loading control.