Table 1.
Eleven DNA extraction methods and descriptive statistics of the measurements used to evaluate the respective quality of DNA extracted.
Table 2.
Descriptive statistics of the measurements used to evaluate quantity of DNA extracted by 11 different methods.
Table 3.
Statistical comparison of 11 genomic DNA extraction methods applied to 16 animals.
Figure 1.
Real-time PCR amplification plot of the gly-A gene, from Campylobacter coli spiked extracted samples from sheep and the controls containing only the Campylobacter coli DNA spike.
Results from one sample with higher Ct value, extracted with the Phenol-Chloroform protocol is also shown indicating the presence of inhibitors.
Figure 2.
Representative results from gel electrophoresis analysis of genomic DNA from two different ovine blood samples extracted by eleven methods.
Charge Switch gDNA Mini Tissue (lanes 1, 2), Nucleospin Blood (lanes 3, 4), Nucleospin Blood-Buffy Coat (lanes 5, 6), Modified Blood (lanes 7, 8), Nucleospin Tissue-Buffy Coat (lanes 9, 10), Modified Tissue (lanes 11, 12), Modified Dx (lanes 13, 14), Nucleospin Blood XL (lanes 15, 16), Phenol-Chloroform (lanes 17, 18), In-house (lanes 19, 20), Nucleospin Blood L (lanes 21, 22), M molecular weight marker l DNA/Hind III digest.
Table 4.
Assessment of consumables cost per sample and process duration of 11 DNA extraction methods.
Figure 3.
Representative results from gel electrophoresis analysis of genomic DNA from thirty different ovine blood samples extracted by four methods.
Modified Blood (lanes 1 to 8), Modified Tissue (lanes 9 to 15), Modified Dx (lanes16 to 22), In-house (lanes 23 to 30), M molecular weight marker l DNA/Hind III digest.
Table 5.
Statistical comparison of four genomic DNA extraction methods based on spectrophotometer measurements (OD260/280nm, OD260/230nm), DNA concentration and Total DNA yield; results are marginal means with standard errors in parentheses pertaining to 600 animals.
Table 6.
Statistical comparison of four genomic DNA extraction methods applied to 600 animals, based on microarray genotyping quality using average call rates across all SNP and individual SNP call rates; marginal means with standard errors in parentheses.