Table 1.
Histological characteristics of human samples per diagnosed category.
Table 2.
Metabolic and histological characteristics of the used NAFLD mouse models.
Figure 1.
Histological photomicrographs of human NASH and NASH in mice.
Liver histological cross-sections from a healthy control subject (A) and a NASH patient (B). Liver histological cross-sections from a healthy control (C) and a NASH E3L.CETP mouse (D). All photomicrographs: Hematoxylin and eosin; magnification 200x.
Table 3.
Histological features of NAFLD in humans and presence or absence in mice.
Figure 2.
Hepatocellular steatosis, hypertrophy and inflammation.
Liver cross-sections are from a NASH patient (A) or a E3L.CETP mouse fed a high fat diet supplemented with cholesterol to induce NASH (B). Macrovesicular steatosis (dotted line arrow): large lipid droplets are present in hepatocytes; microvesicular steatosis (bold arrow): small lipid droplets are present in hepatocytes. Hypertrophy (open arrow): the representative cell is much larger than the surrounding steatotic hepatocytes but has the same cytoplasmic characteristics. Clusters (aggregates) of inflammatory cells (within circles). All photomicrographs: Hematoxylin and eosin; magnification 200x.
Table 4.
Grading system for rodent NAFLD.
Figure 3.
Photomicrograph of cells with collagen staining (Sirius red). Liver histological cross-sections are from a healthy control subject (A) and a NASH patient (B) or a healthy control (C) and a LDLR−/−.Leiden mouse fed a high fat diet to induce NASH (D). All photomicrographs: Sirius red; magnification 100x.
Figure 4.
Bland-Altman plot for correlation analysis between two observers for measurement of macrovesicular steatosis, microvesicular steatosis, hypertrophy and inflammation.
X-axis: average measurements of two observers. Y-axis: Difference between measurements of two observers. UL: Upper 95% limits of agreement. LL: Lower 95% limits of agreement.
Table 5.
Inter-observer reproducibility of NASH histological features according to the mouse NAFLD/NASH scoring system.
Figure 5.
Bland-Altman plot for correlation analysis between two observations of one observer for measurement of macrovesicular steatosis, microvesicular steatosis, hypertrophy and inflammation.
X-axis: average measurements of two observations. Y-axis: Difference between measurements of two observations. UL: Upper 95% limits of agreement. LL: Lower 95% limits of agreement.
Table 6.
Intra-observer reproducibility of NASH histological features according to the mouse NAFLD/NASH scoring system.