Figure 1.
Up-regulation of plasma apoD levels in Western-fed WT mice.
Western blot and semi-quantitative analyses of apoD levels in WT mice fed a 12-week chow and Western diets. Data is representative of both sexes, n = 4–5 per group, mean±SD, *p<0.05.
Table 1.
Lipid parameters in male and female WT and apoD−/− mice after 12 weeks on a Western diet.
Figure 2.
ApoE-HDL enrichment and lipids content changes in HDL particles.
Size exclusion chromatography lipid profiles (total cholesterol, free cholesterol, phospholipids) of pooled plasma from A) female and B) male WT and apoD−/− mice after 12 weeks on a Western diet.
Figure 3.
No change in plasma apoAI, but changes in apolipoproteins content in HDL-size exclusion chromatography fractions.
A) Western blot analysis of apoAI levels from the SEC-HDL fractions. B) Semi-quantitative analysis of plasma apoAI levels. C) SimpleStain and Western blotting for apolipoprotein levels of pooled SEC-HDL fractions (10–20 µg).
Table 2.
Lipoprotein particle size and concentrations (nmol/L) analyzed by IM methodology from pooled plasma of WT and apoD−/− mice fed a Western diet for 12 weeks.
Figure 4.
A) Plasma LCAT and B) PLTP activities from female and male WT and apoD−/− mice on a Western diet for 12 weeks. n = 7–14, values are mean±SD, *p<0.05.
Figure 5.
Decrease in the relative protein expression of SRBI and LDLR in female apoD−/− mice but opposite pattern for mRNA expression.
Liver levels of A) Cholesterol and B) Triglyceride C) Relative expression of genes and D) Protein expression levels of LDLR and SRBI after 12 weeks on a Western diet. n = 5–7 per group, mean±SD, *p<0.05, **p<0.01.
Figure 6.
A decrease in 3H-CE HDL fractional catabolic rate in female apoD−/− mice only.
Mice were fed a Western diet for 12 weeks and 3H-cholesteryl oleate-labeled apoD−/−HDL was injected. A) Changes in plasma 3H-CE-HDL and calculated fractional catabolic rate (FCR), B) 3H-cholesterol (% of injected dose) in the liver, C) 3H-sterol and bile acids (% of injected dose) in feces. The data represent n = 4–6 per group, mean±SD, * indicates statistical significant, p<0.05.