Table 1.
(a) Characteristics of CLL patients and control group. (b) Percentages of plasmablasts and serum anti-pneumococcal antibody as well as IgG2 levels before and after PCV13 vaccination in the CLL patients and control group.
Figure 1.
Specific antipneumococcal antibody titers before and after PCV13 vaccination in: (a) the control group and (b) CLL patients (P<0.001, and P<0.001, respectively).
Proportion of plasmablasts before and after PCV13 vaccination in: (c) the control group and (d) CLL patients (P = 0.001, and P = 0.0266, respectively). Serum IgG2 levels before and after PCV13 vaccination in: (e) the control group and (f) CLL patients (P = 0.0166, and P = 0.012, respectively). A multiparameter flow cytometric analysis of the percentages of plasmablasts before and 7 days after vaccination. Representative example of gating on CD19+ B-cell subpopulations. Percentages of cells in the different quadrants are shown. CD19+/IgD−/CD27++ plasmablasts (upper left quadrant) and CD19+/IgM/CD38++ plasmablasts (lower left quadrant) before vaccination and CD19+/IgD−/CD27++ plasmablasts (upper right quadrant) and CD19+/IgM/CD38++ plasmablasts (lower right quadrant) after vaccination in: (g) the individual CLL patient who did not respond to PCV13 administration and (h) the individual healthy subject from the control group, who responded to PCV13 administration.