Figure 1.
Transmission electron micrograph showing negatively stained Pseudoalteromonas phage B8b.
Figure 2.
One-step growth curve of Pseudoalteromonas phage B8b on Pseudoalteromonas sp. QC-44 strain (▮, total PFU; Ο free PFU).
Figure 3.
Phylogenetic analyses of the bacterial hosts used to test the Pseudoalteromonas phage B8b phage host range.
Bacterial strains infected by siphovirus B8b are labeled in black and the efficiency of phage B8b in hosts infected is indicated. Efficiency is expressed in relative PFU, where the highest was set to 100% and the same phage titer dilution was used for all the bacterial strains (106). Names in brackets are strain designations (See S1 Table for more information).
Table 1.
Genomic annotation of Pseudoalteromonas siphovirus B8b and structural proteomic results.
Figure 4.
Genome structure of Pseudoalteromonas phage B8b represented by 3 contigs and genome comparison with the putative prophage of Marinobacterium stanieri.
Lines drawn between the genomes represent shared sequence similarity, which is given next to each line as percentage amino acid (aa) identity (id).
Figure 5.
Box plots show the percent amino acid (aa) identity for metagenomics reads (32 metagenomes, POV) recruiting to predicted genes from Pseudoalteromonas phage B8b as well as the abundant Pelagiphage HTV0C10P (GenBank No/ KC465898) and the non-marine Enterobacteriaphage T4 (GenBank No. NC_000866).
Metagenomes were grouped in 6 categories product of the combination of photic zone and site location as appear in Hurwitz and Sullivan [25]. A) Percentage of the genome that is being covered by the metagenomics reads. B) Percentage of reads that better align to ORFs in the indicated genome (as per bitscore comparison) than the rest of NR. In box plots boxes mark the upper and lower quartile with the median shown in red, whiskers are extended to 1.5 times the interquartile range, finally, red crosses show outliers.