Table 1.
Bacterial strains used in this study.
Table 2.
Primers and their concentrations in multiplex PCR.
Table 3.
Oligonucleotide probes used in this study.
Figure 1.
Probe positions on the microarray.
OA-1993, the positive control probe based on the 16S rRNA gene. WL-4006, the negative control probe. Cy3, the positional reference and printing control probe. Blank, 50%DMSO. The rest are the specific probes for the target strains.
Figure 2.
Unrooted phylogenetic trees constructed by the neighbor-joining method based on the ITS-tRNAAla and ITS-tRNAIle genes.
Bootstrap values were based on 1,000 replications and only values greater than 50% are shown. A, Unrooted ITS-tRNAAla gene phylogenetic tree of Legionella spp. and subspp. constructed with the neighbor-joining method. B, Unrooted ITS-tRNAIle gene phylogenetic tree of Legionella spp. and subspp. constructed with the neighbor-joining method.
Figure 3.
Microarray differentiation of the Legionella spp.
L. pneumophila; (2) L. anisa; (3) L. bozemanii; (4) L. dumoffii; (5) L. fairfieldensis; (6) L. gormanii; (7) L. jordanis; (8) L. longbeachae; (9) L. micdadei; (10) L. maceachernii; (11) L. anisa and L. dumoffii; (12) L. anisa and L. longbeachae; (13) L. fairfieldensis, L. gormanii and L. jordanis; and (14) L. longbeachae, L. maceachernii and L. micdadei.