Table 1.
Primer pairs selected for RNA-seq validation by qRT PCR.
Table 2.
Pathological characteristics of the HFHSD and control groups after sacrificing the minipigs.
Figure 1.
Extensive inflammatory cell infiltration within the dilated hepatic sinusoids in the HFHSD group (H&E stain).
A: HFHSD group portal area; B: Control group portal area; C: HFHSD group hepatic lobule; D: HFHSD group hepatic lobule. A, B, C, D, bar = 100 µm. Note: a, Ito cell; b, lymphocyte; c, Kupffer cell; d, eosinophil; e, neutrophil.
Figure 2.
The ultrastructure of inflammatory cells in the hepatic sinusoids of the HFHSD group.
There were a large number of phagocytic granules in the Kupffer cells, neutrophils and eosinophils. A: Kupffer cell; B: lymphocyte; C: neutrophil; D: eosinophil; bar = 2 µm.
Figure 3.
Validation of the RNA-Seq data by qRT-PCR.
The 16 genes in minipig livers associated with lipid metabolism, chemokines and the immune response were selected for validation. The data are presented as the fold differences between the HFHSD group and the control group, and each gene was normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) levels.
Table 3.
Summary of the numbers of total reads, matched genes and junction reads of each individual in this study.
Figure 4.
Distribution of the genes according to their expression profiles by RPKM.
Figure 5.
Gene ontology (GO) analysis of the Bama minipig transcriptome data (GO level 2).
GO analysis of 822 genes differentially expressed between the HFHSD group and the control group, which was used to predict the genes' involvement in biological processes (A), molecular functions (B) and cellular components (C). The GO Id and name are shown with different colors.
Figure 6.
List of genes involved in the chemokine signaling pathway.
The genes in the red box represent differentially expressed genes between the HFHSD group and the control group in RNA-seq analysis. All other genes had no obvious differences in this experiment.
Figure 7.
List of genes involved in the natural killer cell-mediated cytotoxicity pathway.
The genes in the red box illustrate differentially expressed genes between the HFHSD group and the control group in the RNA-seq analysis. All other genes had no obvious differences in this experiment.
Figure 8.
List of genes involved in the T cell receptor signaling pathway.
The genes in the red box represent differentially expressed genes between the HFHSD group and the control group in the RNA-seq analysis. All other genes had no obvious differences in this experiment.
Figure 9.
List of genes involved in the leukocyte transendothelial migration pathway.
The genes in the red box illustrate differentially expressed genes between the HFHSD group and the control group in the RNA-seq analysis. All other genes had no obvious differences in this experiment.