Table 1.
Pedigree details of recurrent and donor parents used in the study.
Figure 1.
Structure of the alleles of crtRB1 gene causing variation in β-carotene concentration of maize.
E: Exon; I: Intron; TE: Transposable element. A: No TE insertion at 6th Exon causing favourable allele 1 (543 bp amplicon); B: 325 bp TE insertion at 6th Exon causing unfavourable allele 2 (296+875 bp amplicon); C: 1250 bp TE insertion at 6th Exon causing unfavourable allele 3 (296+1221+1880 bp amplicon).
Figure 2.
Marker-assisted backcross breeding scheme adapted for the introgression of allele 1 of the crtRB1 3′TE gene in to elite parent (V335 and V345) of the maize hybrid Vivek Hybrid-27 (RP: Recurrent Parent; DP: Donor Parent).
Figure 3.
Recurrent and donor inbreds showing polymorphism for alleles of crtRB1 gene.
Table 2.
Distribution of cross-specific polymorphic SSR markers identified and used for background selection.
Table 3.
Segregation pattern of alleles of the crtRB1 gene in different backcross- and selfed- generations across seven crosses.
Figure 4.
Segregation of allele1 and allele 3 in BC2F2 generation (HKI1105×HP467-6) using the crtRB1 gene specific marker (RP: Recurrent Parent; DP: Donor Parent).
Figure 5.
Plant-, tassel- and ear- characteristics of original and β-carotene rich versions of the parental inbreds.
A: VQL1; B: Improved VQL1; C: HKI1105; D: Improved HKI1105; E: HKI161; F: Improved HKI161.
Table 4.
Recovery of recurrent parent genome and kernel β-carotene concentration of the MAS-derived parental inbreds used for reconstitution of hybrids.
Table 5.
Kernel β-carotene concentration and agronomic performance of reconstituted hybrids developed through MAS at Delhi and Dharwad.
Figure 6.
Ear- and grain- characteristics of the original and reconstituted version of hybrid.
A: Vivek Hybrid-27; B: Improved Vivek Hybrid-27.
Table 6.
Agronomic performance of reconstituted hybrids developed through MAS at Delhi and Dharwad.