Figure 1.
A: size and shape, not in scale, of the SMF exposure system showing the values of magnetic induction measured at different points. B: size and shape of a single magnet (not in scale) and allocation of the three magnets (not in scale). C: computer simulation in scale of magnetic flux showing the direction of the field lines. The light grey area indicates higher, while the dark grey lower values of magnetic flux density.
Figure 2.
SMF exposure of cultures showing the measured and simulated magnetic inductions.
A: schematic representation, not in scale, showing the exact localization where the culture flasks have been placed within the SMF exposure system. B: size and shape, not in scale, of a single culture flask. The centre of the bottom of the culture flask has been designated as the origin ‘0’ of the reference system; the x and z axes were arbitrarily chosen, and the y-axis was perpendicular to flask and outgoing up from the same flask. C-E: magnetic induction values measured (spots) and simulated (continuous black line) along the x (C), z (D), and y axes (E) of the culture flask. Each value represents the mean ± SE of six independent experiments, each done in duplicate.
Figure 3.
Viability and LM-based morphology of SH-SY5Y cells.
A: dose-response curve of viability (MTT assay) of SH-SY5Y cells treated with different concentrations of cisplatin (cisPt) (0.1, 0.5 or 1 µM) for 2 h. B: time-course of viability (MTT assay) of 0.1 µM cisPt-treated and/or Static Magnetic Field (SMF)-exposed cells for 2, 4 or 24 h. C: LM images of apoptotic (left) and necrotic (right) cells respectively treated with 0.1 or 1 µM cisPt. D–E: LM images of SH-SY5Y cell cultures at 2 (D) or 24 h (E) of: no treatment (−SMF/−cisPt), treatment with 0.1 µM cisPt (−SMF/+cisPt), SMF-exposure (+SMF/−cisPt) or SMF-exposure combined with 0.1 µM cisPt drug (+SMF/+cisPt). Values are reported as percentage of the control untreated cells considered as 100%. Each value represents the mean ± SE of six independent experiments, each done in duplicate. Single star indicates values significantly different from the respective untreated control cells (p<0.0083). Two stars indicates value significantly different either from 0.1 µM cisPt-treated that from untreated control cells (p<0.0083). Bars = 10 µm.
Figure 4.
TEM micrographs of SH-SY5Y cells undergone for 2 h to different treatments.
A: mitochondria of cells treated with 0.1 µM cisplatin (cisPt). B: mitochondria of cells treated with 1 µM cisPt. C: control cell. D: cell treated with 0.1 µM cisPt (apoptotic cell). E: SMF-exposed cells. F: cell treated with 1 µM cisPt (necrotic cell). G: 0.1 µM cisPt-treated and SMF-exposed cell. Bars = 0.5 µm.
Figure 5.
Western blot of caspase-3 of SH-SY5Y cells.
Cells were exposed for 2, 4 or 24 h to different treatments: no treatment (−SMF/−cisPt), 0.1 µM cisplatin (cisPt) (−SMF/+cisPt), Static Magnetic Field (SMF)-exposure (+SMF/−cisPt) or SMF-exposure plus 0.1 µM cisPt (+SMF/+cisPt). The values are reported as percentage of the control untreated cells considered as 100%. Single star indicates a value significantly different from untreated control cells at the same time point (p<0.0083). Two stars indicates a value significantly different either from cisPt-treated or from untreated control cells at the same time point (p<0.0083). Each value represents the mean ± SE of six independent experiments, each done in duplicate.
Figure 6.
ROS production of SH-SY5Y cells.
A: dose-response curve of ROS production (NBT assay) of SH-SY5Y cells treated with different concentrations of cisplatin (cisPt) (0.1, 0.5 or 1 µM) for 2 h. B: time-course of ROS production (NBT assay) by 0.1 µM cisPt-treated and/or Static Magnetic Field (SMF)-exposed cells for 2, 4 or 24 h. The values are reported as percentage of the NBT reduction of control untreated cells considered as 100%. Single star indicates values significantly different from the respective untreated control cells (p<0.0083). Two stars indicates value significantly different either from 0.1 µM cisPt-treated that from untreated control cells (p<0.0083). Each value represents the mean ± SE of six independent experiments, each done in duplicate.