Figure 1.
Micrographs of two vesicle populations with different vesicle densities in solution.
Vesicle abundance is higher in (a) than in (b). The lower abundance of vesicles (b) is preferred for better performance of the computer vision algorithms.
Figure 2.
Scheme of the perfusion chambers (a).
Silicone model with four perfusion chambers named C1–C4 (b). The side view is given in the figure and the depth of the chambers is 0.5 mm. A single chamber with two tracks (P1 and P2) locations of two tracks recorded in each chamber (c). The two tracks together cover approximately 3% of the chamber area.
Figure 3.
Light micrograph of vesicles in a micrograph (a), vesicles with overlaid segmentation (b), mask with segmented vesicles separated from the background in which each vesicle is marked with a number (c).
Table 1.
Vesicle measurements from three experiments; the experimental parameters for each experiment (time is the duration of incubation at recording; the number of chambers is the same as the number of samples).
Figure 4.
Changes in samples from different vesicle populations over time (from 3 to 60 minutes).
Each experiment was conducted using a new initial vesicle population and each sample point represents a single chamber. Vesicle quantities (a), mean projected diameter sizes (b), and mean isoperimetric quotients (c). The box plot consists of mean minimal and maximal values 25th 50th and 75th percentile.
Figure 5.
Histograms of vesicle diameter size distribution (a) and vesicle isoperimetric quotient (b).
Data samples for both histograms were taken from one randomly selected sample and their shapes are representative of all samples.