Figure 1.
SDS-PAGE analysis of the purified MnP from Irpex lacteus CD2.
lane M: molecular mass marker; lane 1 and lane 2: purified MnP.
Table 1.
Purification of manganese peroxidase from Irpex lacteus CD2.
Figure 2.
UV-visible spectrum of the purified CD2-MnP.
Figure 3.
Effect of pH and temperature on the activity and stability of purified CD2-MnP from Irpex lacteus CD2.
A: Effect of pH on MnP activity. The activity of 100% was that which was measured at the optimal pH. B: Effect of pH on the stability of CD2-MnP. The initial MnP activity before incubation was set as 100%. C: Effect of temperature on MnP activity. The activity of 100% was that which was measured at the optimal temperature. D: Effect of temperature on the stability of CD2-MnP. The initial MnP activity before incubation was set as 100%.
Figure 4.
Effect of metal ions and organic solvents on the activity of purified CD2-MnP.
A: The effect of different metal ions on MnP activity. The MnP activity of the control without adding any metal compound was set as 100%. B: The effect of different organic solvents on MnP activity. The MnP activity of the control without adding any organic solvent was set as 100%.
Table 2.
Effect of metal ions on the stability of purified CD2-MnP from Irpex lacteus CD2.
Table 3.
Effect of organic solvents on the stability of purified CD2-MnP from Irpex lacteus CD2.
Figure 5.
Decolorization of different types of dyes by the purified CD2-MnP with the coexistence of metal ions.
The reaction mixture in a total volume 1 ml contained (final concentration): malonate buffer (20 mM, pH 4.5), Mn2+ (1.6 mM), H2O2 (0.08 mM), purified CD2-MnP (0.25 U/ml), dye (50 mg/L) and Ca2+, Co2+, Mg2+, Zn2+ (4 mM). CK (MnP+H2O2) was the control without addition of any metal compound except Mn2+. H2O2 (no MnP) was the negative control without addition of purified CD2-MnP. (A): Decolorization of RBV5R; (B): Decolorization of DR5B; (C): Decolorization of RBBR; (D): Decolorization of IC; (E): Decolorization of MG. RBV5R: Remazol Brilliant Violet 5R, DR5B: Direct Red 5B, RBBR: Remazol Brilliant Blue R, IC: Indigo Carmine, MG: Methyl Green. The negative control (H2O2 was added into the decolorization mixture in the absence of purified CD2-MnP) showed no significant decolorization of different dyes.
Figure 6.
Decolorization of different types of dyes by the purified CD2-MnP with the coexistence of organic solvents.
The reaction mixture in a total volume 1 ml contained (final concentration): malonate buffer (20 mM, pH 4.5), Mn2+ (1.6 mM), H2O2 (0.08 mM), purified CD2-MnP (0.25 U/ml), dye (50 mg/L) and methanol, DMSO, ethylene glycol, glycerin (20%). CK (MnP+H2O2) was the control without addition of any organic solvent. (A): Decolorization of RBV5R; (B): Decolorization of DR5B; (C): Decolorization of RBBR; (D): Decolorization of IC. (E): Decolorization of MG. RBV5R: Remazol Brilliant Violet 5R, DR5B: Direct Red 5B, RBBR: Remazol Brilliant Blue R, IC: Indigo Carmine, MG: Methyl Green.
Table 4.
Decolorization of simulated textile wastewater (10%, 30%, 50%) by purified CD2-MnP for 72 h.