Figure 1.
Multivariate discriminant analysis of the UBE2D2 normalised gene GeXP hCellMarkerPlex data from human colon normal (white triangle) (n = 24), adenomatous polyp (grey triangle) (n = 17) and carcinoma (black triangle) (n = 19) tissues.
Information on the gene symbols on the biplot is available in Table S2. (A) Principal component analysis (PCA) biplot permits visualisation of inherent clustering patterns of individual tissue samples and associated gene expression levels. (B) Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA) was applied to fit a 2-class supervised model maximising covariance and discriminating gene expression profiles associated with the different tissues sample types; the biplot shows scores and loadings as well as the regression coefficients best explaining each class ($M4.DA(N),$M4.DA(A),$M4.DA(C). (C) Rank of importance of cell marker genes within the OPLS-DA. (D) Matrix showing the associated misclassification rates.
Figure 2.
Relative gene expression levels in human colon normal, adenomatous polyp and carcinoma tissue generated using the GeXP hCellMarkerPlex assay.
Gene expression is normalised to internal reference gene UBE2D2 in the hCellMarkerPlex. The letters indicate significant (p<0.05) difference in gene expression between ‘a’ normal (n = 24) and either adenomatous polyp (n = 17) or carcinoma (n = 19), ‘b’ normal, adenomatous polyp and carcinoma, ‘c’ normal and carcinoma, ‘d’ normal and adenomatous polyp and ‘e’ carcinoma and either normal of adenomatous polyp.
Figure 3.
Gene expression of (A) long and (B) short form variants of MS4A12 in human colon normal, adenomatous polyp and carcinoma tissue.
Gene expression is normalised to reference gene UBE2D2. The asterisk (*) indicates significant decrease in expression levels of adenomatous polyp compared to normal, p<0.005. (C) – (D). In situ hybridisation of MS4A12 transcripts in human colon (C) normal, (D) adenomatous polyp and (E) carcinoma. Emulsion autoradiographs showing expression of MS4A12 at luminal epithelial surface (ep) of normal (C) in bright field and corresponding dark field images in antisense (left) and sense (right) hybridised tissue sections (n = 5). MS4A12 is largely absent in adenomatous polyp (D) and localised in discrete areas of epithelium in carcinoma (E). Bar = 20 µm.
Figure 4.
In situ hybridisation of LGR5 transcripts in human colon (A) normal, (B) adenomatous polyp and (C) carcinoma.
Emulsion autoradiographs showing expression of LGR5 in discrete single cells (arrow) in epithelium (ep) in normal (A) and extensive expression in epithelium (ep) of adenomatous polyp (B) and carcinoma (C) in bright field and corresponding adjacent dark field images. Antisense hybridised tissue sections are shown to the left with sense hybridised tissue sections adjacent to the right (n = 5). Bar = 20 µm.
Figure 5.
Localisation of CDX2 transcripts and encoded protein in human colon.
In situ hybridisation of CDX2 transcripts in human colon (A) normal, (B) adenomatous polyp and (C) carcinoma. Emulsion autoradiographs showing expression of CDX2 in epithelium (ep) in bright field and corresponding adjacent dark field images in antisense (left) and sense (right) hybridised tissue sections (n = 5). Bar = 20 µm. (D) – (F) Representative paraffin-embedded tissue sections show immunohistochemical localisation of CDX2 expression in the human colon epithelium in (D) normal, (E) adenomatous polyp and (F) carcinoma. (G) Semi-quantitative scoring of staining intensity (increasing from + to +++) revealed increased immunostaining for CDX2 in adenomatous polyp and carcinoma (n = 8). Scoring system: + (detectable nuclear staining, weak), ++ (easily visible nuclear staining), n/a – no adenoma tissue in histological section.
Figure 6.
In situ hybridisation of NOX1 transcripts in human colon (A) normal, (B) adenomatous polyp and (C) carcinoma.
Emulsion autoradiographs showing expression of NOX1 in epithelium (ep) in bright field and corresponding adjacent dark field images in antisense (left) and sense (right) hybridised tissue sections (n = 5). Bar = 20 µm.
Figure 7.
In situ hybridisation of SLC9A2 transcripts in human colon (A) normal, (B) adenomatous polyp and (C) carcinoma.
Emulsion autoradiographs showing expression of SLC9A2 in epithelium (ep) in bright field and corresponding adjacent dark field images in antisense (left) and sense (right) hybridised tissue sections (n = 5). Bar = 20 µm.