Figure 1.
Schematic representation of the sarcomere cytoskeleton distances.
A, an illustration of the sarcomere elementary parts; B, a sarcomere image by electron microscopy; C, a SHGM image of a myofibril; and D, a SHGM intensity profile along the myofibril showing the distances measured to characterize the sarcomere cytoskeleton: sarcomere length (SL), intra-sarcomeric A-band lengths (ABL) and thick-thin filament interaction length (TTIL).
Table 1.
Fetal biometric and echocardiographic results in IUGR and control fetuses.
Figure 2.
Ultrastructural sarcomere changes in fetal hearts from IUGR and controls.
A, A representative SHGM image from unstained fetal rabbit left ventricle. The sarcomeres are clearly delimited by thick black lines (Z-discs) and the SHGM signal originates from the thick myosin filaments (in green). In the central region of the myosin filaments appears a thinner black line, identified as the M-band. Scale bar = 20 µm. B and C, show average distances between two consecutive Z-discs (SL) and intrasarcomeric A-bands (ABL), respectively. D, shows thick-thin filament interaction length (TTIL), as the mean width of the A-band-related peaks. Data are expressed as mean ± SD.
Figure 3.
Ultrastructural sarcomere changes in adult hearts from IUGR and controls.
A, Representative SHGM image from unstained adult rabbit left ventricle. Scale bar = 20 µm. B and C, show average distances between two consecutive Z discs (SL) and two intrasarcomeric A bands (ABL), respectively. D, shows the length of thick-thin filament interaction length (TTIL). Data are expressed as mean ± SD.
Figure 4.
Acyclic graph showing the M-line cellular component significantly over-represented (in red) in IUGR compared to healthy control hearts. In the GO hierarchy, biological knowledge can be represented as a tree where functional terms near the root of the tree make reference to more general concepts while deeper functional terms near the leaves of the tree make reference to more specific concepts. If a gene is annotated to a given level, then is automatically considered to be annotated at all the upper levels up to the root.
Table 2.
Results from most relevant sequences included in M-band functional class identified by FatiScan gene set analysis (M-band (GO: 0031430) block of genes).