Figure 1.
An artificial indel region in the alignment of cox1 sequences between the Folmer region and the C1-J-2183/C1-N-2776 extension.
Such indel region arising due to incomplete or poor reads, commonly hampers the accuracy of phylogenetic analyses.
Table 1.
Common cox1 primers used in arachnid systematics, and tested in this study.
Figure 2.
Fifteen selected spider species (see Table 2) representing the major phylogenetic lineages on a simplified phylogeny [35].
Table 2.
The success rate of different primer combinations for the fifteen selected spider species varies from 100% to 30%.
Figure 3.
Gel images showing different success rates in cox1 amplification using the ten tested primer pairs.
Figure 4.
The new primer C1-J-2123 binding site is 60 bp upstream of the C1-J-2183 binding site.
Figure 5.
The newly amplified and elongated C1-J-2123/C1-N-2776 sequence overlaps with the Folmer (LCO1490/HCO2198) sequence.