Table 1.
The primers used in this study.
Figure 1.
The fiber color, yield, quality and microstructure of Z128 and Z263.
Statistical analyses were performed at 95% condence with IBM SPSS Statistics 11.0 (SPSS Inc., Chicago, USA). Values with an asterisk represented a significant differe- nce at P<0.05. The fiber microstructure of Z263 (C I) and Z128 (C II) was observed under a microscope (×3000 and ×2500, respectively).
Figure 2.
All-unigenes classified by GO analysis.
Figure 3.
COG classification of all-unigenes.
A–Z represented different functions classified by GO analysis, respectively.
Figure 4.
Mapping of all expressed unigenes.
This figure was created by comparing gene expression levels of Z128 to Z263. FDR≤0.001, |log2Ratio| ≥1 was used as threshold, red dots represented up-regulated genes, green dots represented the down-regulated genes, and blue dots represented gene expression with no significant difference.
Table 2.
The DEGs enriched terms in GO analysis (P-value<1).
Table 3.
The top 10 DEGs enriched pathways in KEGG analysis.
Table 4.
The involved unigenes in Flavone and flavonol biosynthesis and Anthocyanin biosynthesis pathways.
Figure 5.
The schematic of pigment formation in cotton fiber.
The red and green arrows indicated the up- and down-regulated status detected by comparing gene expression in Z128 with that in Z263. chalcone synthase (CHS); chalcone isomerase (CHI); flavanone 3-hydroxylase (F3H); flavonol synthase (FLS); dihydroflavonol 4-reductase (DFR); leucoanthocyanidin reductase (LAR); anthocyanidin synthase (ANS); UDP-favonoid glucosyl transferase (UFGT); anthocyanidin reductase (ANR); anthocyanin 5-O-glucosyltransferase (5GT).
Table 5.
Fiber development related DEGs of brown and white fibers.