Table 1.
The final population density (N) of species of mites on antibiotic additive diets and fitted concentration EC50 is an effective concentration of antibiotic s in diets for 50% population growth reduction in the comparison to growth on control diet.
Figure 1.
The effect of antibiotics pre-treatment on the final population size after 21 days of growth on untreated diet, averages with respective standard deviations.
The Dunnet significant differences from the control are marked by asterisk; CON - untreated control, AMP –ampicillin, NEO – neomycin, STR – streptomycin.
Figure 2.
Venn’s diagram comparing the sequences in 16S rRNA library of the clones (A) and isolates (B) from mite on control and antibiotic treated diets.
Table 2.
The analyses of 16S rRNA libraries from the clones from stored product mites reared on control diets.
Table 3.
The analyses of 16S rRNA libraries from isolates from stored product mites reared on antibiotic treated and control diets.
Figure 3.
Quantitative real-time PCR of total bacteria from the DNA extracted from stored product mites reared on control and antibiotic treated diets.
The numbers of copies were recalculated per one specimen, averages with respective standard deviations. The Dunnet significant differences from the control are marked by asterisk; CON - untreated control, AMP –ampicillin, NEO – neomycin, STR – streptomycin.
Figure 4.
The comparison of cloned 16S rRNA sequences of stored product mites (Acarus siro, Lepidoglyphus destructor and Tyrophagus putrescentiae) on control and antibiotic supplementation (1 mg g−1 of diet) diets.
Figure 5.
The comparison of 16S rRNA sequences from isolated bacteria from of stored product mites (Acarus siro, Lepidoglyphus destructor and Tyrophagus putrescentiae) on control and antibiotic supplementation (1 mg g−1 of diet) diets.