Figure 1.
Schematic presentation of the workflow of the second SPIDIA-RNA EQA.
Blood was drawn from two donors (D1, D2) into separate EDTA containing bags. EST = Evacuated Secondary Tube, that does not contain any chemical formulation. T0 = Blood processed without storage, immediately after blood collection. day 1, day 2 = Time period between blood collection and RNA preparation.
Figure 2.
Relative Transcript levels of FOS and IL1B in individual and pools samples.
Overall distribution of FOS (A) and IL1B (B) according to time storage. Each dot represents the expression levels of each individual (black) or pool (gray) samples for each time; the dashed lines indicate the time-trend for each sample. The continuous lines indicate the overall trend for individual (black) and pool (gray) samples. The horizontal dot-dashed line indicates the expected value of T0.
Table 1.
Classification of the proficiency of the laboratories.
Figure 3.
Blood collection tube and/or storage temperature and Gene Expression.
Overall distribution of FOS and IL8 according to blood collection tube (3A and 3C, respectively) and to storage temperature/collection tube in RNA D (3B and 3D, respectively). The box horizontal sides identify the 25th and 75th centile, the horizontal line inside the box the median, the two whiskers correspond to minimum and maximum, and the dashed line indicates the T0 value zero.