Figure 1.
Stages and instruments of the methodological procedures.
In A, removal of meninges and blood vessels. B, dissection of the right olfactory bulb (OB) and tract (OT). C, fractionation in a tissue homogenizer. D, DAPI-stained nuclei in the Neubauer chamber. E, NeuN-positive nuclei. The yellow arrows show nuclei that colocalize DAPI and NeuN, therefore considered to be neuronal, and counted as such.
Table 1.
Characteristics of the sample.
Figure 2.
Average number of cells in the olfactory bulb of men and women.
Blue bars indicate the mean number of cells in males, pink bars for females. Significant differences were found for the total number of cells, as well as for the number of neurons and non-neuronal cells. In all cases, women outnumbered men. Error bars indicate standard deviation. * p<0.05; ** p<0.01.
Figure 3.
Olfactory bulb mass and cell density in men and women.
In A, mean mass of olfactory bulbs for each sex. No difference was found between males and females. In B, mean neuronal, non-neuronal and total density in men and women. Blue bars represent males, pink bars for females. Error bars indicate standard deviation. **p<0.01; ***p<0.005.
Figure 4.
Ratio between non-neuronal and neuronal cells.
No difference was found between men and women (p = 0.837). The bars indicate the mean for males (blue bar) and females (pink bar); error bar represents standard deviation.