Table 1.
Experimental Animal Groups.
Figure 1.
Following decapitation, the brain was exposed and the hippocampus immediately dissected as shown. The whole process takes less than 3 min.
Figure 2.
Effect of One or Several Sertraline Doses on Pro-inflammatory Cytokines Messengers Expression in the Hippocampus.
Relative IL-1β/β-actin (A) and TNF-α/β-actin (B) mRNA expression measured by RT-PCR in the hippocampi of animals administered with (left bars): a single injection of vehicle (G1) or 0.75 mg/kg sertraline (G2); and in animals administered with (right bars): 7 injections for one week of vehicle (G3) or 0.75 mg/kg sertraline (G4). Results are the mean ± SEM values of 6 (G1 and G3), 4 (G2), or 3 (G4) animals per group. *, p = 0.01 and ***, p<0.001 between the dashed line connecting experimental conditions.
Table 2.
Effect of One and Seven Sertraline Low Doses on 4-AP-Induced Seizures.
Figure 3.
Effect of One or Several Sertraline Doses on the Increase in IL-1β and TNF-α mRNA Expression Induced by 4-AP in the Hippocampus.
Relative IL-1β/β-actin (A) and TNF-α/β-actin (B) mRNA expression in the groups administered with a single injection of vehicle (G1) or with one daily injection of vehicle for one week (G3), and in groups injected with 2.5 mg/kg 4-AP and pre-administered with: a single injection of vehicle (G5), a single injection of 0.75 mg/kg sertraline (G6), or one daily injection of 0.75 mg/kg sertraline for one week (G7). Results are the mean ± SEM values of 6 (G5) or 4 (G6 and G7) animals per group.*, p = 0.01 and ***, p<0.001 between the dashed line connecting experimental conditions.
Table 3.
Effect of Sertraline at Two Doses on PTZ-Induced Seizures.
Figure 4.
Effect of Sertraline on the Increase in IL-1β and TNF-α mRNA Expression Induced by PTZ in the Hippocampus.
Relative IL-1β/β-actin (A) and TNF-α/β-actin (B) mRNA expression in the group administered with the single injection of vehicle (G1), and in groups injected with 50 mg/kg PTZ and pre-administered with single injections of: vehicle (G8), 2.5 mg/kg sertraline (G9), or 25 mg/kg sertraline (G10). Results are the mean ± SEM values of 7 (G8) or 5 (G9 and G10) animals per group. *, p<0.04; **, p<0.01 and ***, p<0.001 between the dashed line connecting experimental conditions.
Figure 5.
Effect of Sertraline on the Increase in IL-1β and TNF-α mRNA Expression Induced by LPS in the Hippocampus.
Relative IL-1β/β-actin (A) and TNF-α/β-actin (B) mRNA expression in the group injected with vehicles (G1) and in groups inoculated with 100 µg/kg LPS and pre-administered vehicles (G11), or 2.5 mg/kg sertraline (G12). Results are the mean ± SEM values of 5 (G11) or 4 (G12) animals per group. **, p<0.01 and ***, p<0.001 between the dashed line connecting experimental conditions.