Figure 1.
Characterization of HA coated SR.
A, Optical microscopy pictures of the three kinds of samples (Left, SR; Middle, HA-1/SR; Right, HA-2/SR); B, SEM pictures of the three samples(Left, SR; Middle, HA-1/SR; Right, HA-2/SR); C, X-ray photoelectron spectroscopy of the three samples; D, FTIR of the three samples; E, Dumbbell shape of the sample for the mechanical properties study; F, Mechanical properties of the three samples (Shore hardness; elongation at breaking; tensile stress). Each number has six repeat, the average and stander standard error of the mean was used.
Table 1.
Water contact angle of the three kinds of SR surface (n = 6, ±s).
Table 2.
Surface roughness of the three kinds of SR surface (n = 6, ±s, * P<0.05).
Table 3.
Chemical composition (%) according to XPS analysis.
Figure 2.
Cell compatibility of HA coated SR.
A, Cytotoxicity of materials according to Hoechst 33342 double staining with PI (Left, SR; Middle, HA-1/SR; Right, HA-2/SR); Cell skeleton structure staining of each group (Left, SR; Middle, HA-1/SR; Right, HA-2/SR) (the scale bar is 25 µm); SEM images further demonstrated a more fibroblastic appearance of fibroblasts cultured on HA/SR (the scale bar is 20 µm); B, Cell proliferation of each group studied by CCK-8 (n = 6, *p<0.05); C, Cell area of each group studied by IPP6 (n = 6, *p<0.05).
Table 4.
ISO 10993-5:1999 standard of cytotoxicity evaluation of biomaterials according to RGR.
Table 5.
Cell growth condition of SR and HA/SR (n = 6, ±s, * P<0.05).
Figure 3.
Detection of cell adhesion molecules.
A, Immuno-fluorescence of the cells on the three kinds of SR (the scale bar is 20 µm); B, Western-blot of the cells on the three kinds of SR; C, Optical density ratio of Western-blot; D, Realtime PCR of the cells on the three kinds of SR (n = 6, *p<0.05).
Figure 4.
The mechanism of improved cell adhesion on the HA coated surface of SR.
A, mass result; B, SDS-PAGE picture (1, 2, 3 represent the selected protein points); C, western-blot verification; D, Cell morphology of EF1β interference human dermal fibroblasts on the three kinds of SR (the scale bar is 50 µm) (wild type means common human dermal fibroblasts, EF1Bi means EF1β interference human dermal fibroblasts).