Table 1.
Population characteristics in euthyroid individuals for serum thyroid stimulating hormone (TSH) levels and demographics in the eMERGE Network.
Figure 1.
Manhattan plot of tests of association with serum thyroid stimulating hormone (TSH) levels in euthyroid European Americans in eMERGE.
Data shown are p-values from single SNP tests of association with serum TSH levels in a model adjusted for age, sex, principal component (PC1), and body mass index in euthyroid European Americans in eMERGE Network (n = 4,501). The y-axis represents the –log10 (p-value); horizontal lines represent Bonferroni corrected significance level (p<5×10−08) (top) and suggestive significance level (1×10−04) (bottom). Chromosomes are arranged on the x axis.
Table 2.
Genome-wide significant SNP associations for serum thyroid stimulating hormone (TSH) levels in eMERGE euthyroid European Americans (n = 4,501).
Figure 2.
Comparison of most significant associations identified in European Americans with African Americans from the eMERGE Network.
We plotted p-values, coded allele frequencies, and betas for euthyroid European Americans (n = 4,501) and African Americans (n = 351) in the eMERGE Network for serum TSH level tests of association using SynthesisView. Data shown are comparisons between European Americans (blue markers) and African Americans (red markers) for p-values (data shown are –log10 (pvalue)), genetic effect magnitudes (beta), and minor (coded) allele frequencies (MAF) for the 31 most significant SNPs in European Americans. Red horizontal line on p-value track indicates p = 0.05. SNPs are oriented across the top of the figure, arranged by chromosomal location. Large triangles represent p-values at or smaller than 5×10−08. Direction of the marker for p-values indicates direction of effect for each SNP.
Table 3.
Comparison of associations in eMERGE European Americans to previously published genetic associations with serum thyroid stimulating hormone (TSH) levels.
Figure 3.
Body mass index as a modifier of serum thyroid stimulating hormone (TSH) levels genetic associations in eMERGE European Americans.
Interaction analyses were performed using the SNPs with p<1×10−04 significance levels in the model adjusted for age, sex, PC1, and body mass index in European Americans (n = 4,501). For each significant (p<0.05) interaction term, the model was then stratified by normal/overweight BMI (normal BMI = 18–24.9; overweight BMI ≥25). We considered a SNPxBMI interaction significant at a threshold of p<0.05. Shown are p-values from Wilcoxon rank-sum test comparing median TSH values between BMI categories at each genotype.