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Figure 1.

(a) Shows the low magnification TEM image of the ZnO-NPs, (b) HR-TEM image shows the difference between two lattice fringes, which is about 0.265 nm and consistent with The HR-TEM observations, which indicate the crystallinity of the synthesized products. (c) Typical XRD pattern of grown ZnO-NPs. whereas (d) shows the AFM image of ZnO-NPs.

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Figure 1 Expand

Figure 2.

(a) Inhibition on P.aeruginosa biofilm formation at different concentration of ZnO-NPs. (b) ZnO-NPs induced oxidant generation in bacterial cells treated with various concentrations of ZnO-NPs (0, 5, 10, 25, 50 and 100 µg/mL) incubated for 24 h.

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Figure 2 Expand

Figure 3.

Bio-TEM images of: (a) P.aeruginosa (b) P. aeruginosa with ZnO-NPs.

(c-f) Possible proposed pictorial mechanism.

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Figure 3 Expand

Table 1.

Zone of inhibition of Bacterial growth of P.aeruginosa with increasing concentration of ZnO-NPs done in triplicate manner.

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Table 1 Expand

Figure 4.

(a) The absorption spectra of nanoparticles of ZnO, control and nanoparticles of ZnO with control (P.aeruginosa). (b) Optimizion of pH range (2 to 8.0).

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Figure 4 Expand

Table 2.

Statistical analytical parameter for the determination ofZnO-NPs.

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Table 2 Expand

Figure 5.

(a) pH (6.86) volume graph, (b) Linear calibration graph of nanoparticles (NPs) with (P.aeruginosa) bacteria.

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Figure 5 Expand

Table 3.

Test of precision of the proposed method for ZnO-NPs.

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Table 3 Expand

Figure 6.

Possible schematic presentation for the formation of ZnO-NPs.

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Figure 6 Expand