Table 1.
Patients’ characteristics (n = 135).
Figure 1.
Determination of the detection limit of the D-PCR assay for T790M mutation.
The assay panel included 765 chambers. A chamber harboring both red signal and blue signal represented T790M positive ones. Only blue signal in the chamber represented negative T790M mutation. This figure referred to the panel based status of T790M mutation when the mixed DNA (T790M mutant DNA fragments and internal control DNA fragments) with dilution concentration of 1∶3000.
Table 2.
Comparison of T790M detected by ARMS and digital PCR in pre- and post-EGFR-TKI plasma samples.
Figure 2.
Survival analysis on EGFR-TKI according to the quantity of EGFR T790M mutation.
Kaplan-Meier curves of (A) PFS and (B) OS according to the quantity of de novo EGFR T790M mutation identified by D-PCR in patients harboring sensitizing EGFR mutations (19del or 21L858R) (n = 83). The quantity of de novo T790M mutation was divided to three classes (high, low and nil), which referred to the quantity of de novo T790M mutation >5%, 0–5%, and 0%, respectively.
Figure 3.
Survival analysis on EGFR-TKI according to the dynamic change of EGFR T790M mutation.
Kaplan-Meier curves of (A) PFS and (B) OS according to the dynamic changing tendency of EGFR T790M quantity from pre- to post-EGFR-TKI treatment in patients with both T790M in pre- or post-EGFR-TKI plasma samples and sensitizing EGFR mutations (19del or 21L858R) in initial diagnosis tissue samples (n = 53).
Table 3.
The variation tendency of T790M mutation from pre- to post-TKI in plasma cf-DNA.