Figure 1.
(a) Light microscopy image of a typical transverse cross section of the bone created by stitching together of many individual images. Classification of voids was performed on based on their size. (b) An individual image from the cross sectional image (marked by a white rectangle in image a). (c) Each image was first binarized, separating it into ‘bone’ (white) and ‘void’ (black) (right image). Cavities within the range of 9–50 µm2 were considered to be lacunae (long arrows), cavities within the range of 151–2000 µm2 were considered to be Haversian canal (short arrows) and cavities larger than 2000 µm2 were considered resorptive lesions (arrowheads).
Figure 2.
Light microscopy images of three transverse cross-sections of the femoral mid-diaphysis of (a, c, e) cats with CKD and (b, d, f) healthy cats.
Note dramatic increase in unfilled resorption cavities in CKD cats compared to the healthy cats.
Figure 3.
Dot plots depicting the data of CKD and control cats; the horizontal line represents the median.
(a) resorption cavity density (b) porosity, (c) BMD and (d) Young's modulus.
Table 1.
Morphometric characteristics of cortical bone of the distal femur in CKD and healthy controls by light microscopy.
Table 2.
Morphometry of cortical bone of the mid-diaphyseal femur in CKD and healthy controls.
Table 3. Mechanical properties of the cortical bone of CKD and control groups.
Figure 4.
Trabecular bone analysis: Trabecular thickness (a) and bone volume/total volume (BV/TV, b) in the cancellous bone of CKD and control cats for both the femora (F) and the vertebrae (V).
Data are presented as dot plots. The horizontal line represents the median. Data from the femur and vertebra are similar within the study groups; however, there is a significant difference for both parameters between the study and the control groups. Micro-CT scans of cancellous bone of a control (c) and CKD (d) cat.