Figure 1.
In vitro seed culture, seedling development and reintroduction of Renanthera imschootiana Rolfe.
(A) Stage 0, seed under scanning electron microscopy, ungerminated. (B) Stage 1, testa ruptured. (C) Stage 2, appearance of rhizoids. (D) Stage 3, emergence and elongation of first leaf. (E) Stage 4, one leaf and root present. (F) Stage 5, presence of two or more leaves. (G) Proliferation of PLBs on quarter-strength MS (1/4 MS) medium supplemented with 1.0 mg l−1 BA, 1.0 mg l−1 NAA, 1.0 g l−1 peptone and 20% CW. (H) Differentiation of PLBs on 1/4 MS medium supplemented with 1.0 mg l−1 NAA, 1.0 g l−1 peptone, 100 g l−1 BH, and 1.0 g l−1 AC. (I) Development of seedlings on Hyponex N016 medium supplemented with 0.5 mg l−1 NAA, 1.0 g l−1 peptone, 150 g l−1 BH, and 1.0 g l−1 AC. (J) Transplanted plantlets 6 months after acclimatization in the greenhouse. (K) Reintroduced flowering plantlets from in vitro-derived seedlings on the trunk of Pinus massoniana on Huolu Mountain, Guangzhou. (L) Reintroduced plantlets from in vitro-derived seedlings at the Orchids Garden, South China Botanical Garden. Bars: (A) 50 µm, (B) 100 µm, (C) 0.05 mm, (D) 0.35 mm, (E) 0.40 mm, (F) 0.60 mm, (G, H) 3.0 cm, (I) 4.0 cm, (J, K) 5.0 cm, (L) 3.0 cm.
Table 1.
Seed germination and seedling developmental growth stages of Renanthera imschootiana (modified from Zeng et al., 2012).
Table 2.
Effect of basal medium supplemented with 0.5 mg l−1 NAA, 10 g l−1 sucrose and 1.0 g l−1 AC on germination and development of Renanthera imschootiana 150 DAP seed after 75 days in culture.
Figure 2.
Effect of the degree of seed maturity on in vitro germination of Renanthera imschootiana on quarter-strength MS medium supplemented with 0.5 mg l−1 NAA, 20% CW, 1.0 g l−1 peptone, 10 g l−1 sucrose and 1.0 g l−1 AC 75 days after culture.
Different letters indicate significant differences between days at P<0.05 (DMRT).
Figure 3.
Effect of sucrose concentration on in vitro germination of Renanthera imschootiana on quarter-strength MS medium supplemented with 0.5 mg l−1 NAA, 20% CW and 1.0 g l−1 AC 75 days after culture.
Different letters indicate significant differences between days at P<0.05 (DMRT).
Table 3.
Effect of organic amendments on seed germination of Renanthera imschootiana at 150 DAP on 1/4 MS containing 0.5 mg l−1 NAA, 10 g l−1 sucrose and 1.0 g l−1 AC for 75 days in culture.
Table 4.
Effect of BA concentration on proliferation of Renanthera imschootiana PLBs on 1/4 MS medium with 0.5 mg l−1 NAA, 1.0 g l−1 peptone, 10 g l−1 sucrose, and 20% CW after 60 days in culture on the 8th sub-culture.
Table 5.
Effect of NAA and banana homogenate (BH) concentration on differentiation of Renanthera imschootiana PLBs on 1/4 MS medium containing with 1.0 g l−1 peptone, 10 g l−1 sucrose, 10% CW and 1.0 g l−1 AC after 60 days in culture.
Table 6.
Effect of NAA and banana homogenate (BH) concentration on Renanthera imschootiana plantlet growth on Hyponex N016 medium with 1.0 g l−1 peptone, 20 g l−1 sucrose, 10% CW and 1.0 g l−1 AC after 60 days in culture.
Table 7.
Survival of Renanthera imschootiana plantlets grown on different substrates after transplanting for 60 days.
Table 8.
Survival rates of different ages of transplanted Renanthera imschootiana seedlings after 360- or 720-day reintroduction.