Table 1.
Univariate analysis of the demographics and clinical parameters associated with false-positive results in the laboratory diagnosis of norovirus infections using RT-PCR targeting the RdRp gene.
Figure 1.
Phylogenetic analysis of viral protein 1 sequences from 154 strains of norovirus.
Footnote of Figure 1: A total of 11 reference norovirus strains were included in the analysis. Except for strains of GII3 Wuhan Z1353 CHN2010, GII 6 CMH-N00811 THA 2006 and GII 233 JPN 2007, the other 8 reference strains were close related to the GII 4 Sydney strains.
Figure 2.
Gel electrophoresis of PCR products by RT-PCR amplification of RdRp gene in true-positive samples and false-positive samples.
Footnote of Figure 2: The 5 true-positive samples were confirmed by viral protein 1 gene amplificaion and sequence of amplicons. In the 5 false-positive samples, the amplicon sequences were belonged to human DNA.
Figure 3.
Flow chart of patients undergoing norovirus detections and selection of infection episodes for the identification of factors associated with false-positivity.
Table 2.
Factors associated with false-positive results in the laboratory diagnosis of norovirus infection using RT-PCR targeting the RdRp gene, as determined by multiple logistic regression analysis.