Table 1.
Panel of Leishmania strains used for screening of RAPD markers.
Table 2.
Homology analysis of the sequences of the cloned RAPD markers in 3 Leishmania genomes.
Figure 1.
RAPD profiles obtained with primers OP-O7 (A) and OP-O13 (B) to illustrate examples of monomorphic and polymorphic profiles.
Laboratory codes of the strains are indicated for each lane. SD: Sudan; TN: Tunisia; M: 100 bp DNA size marker. Indicated sizes are in bp.
Figure 2.
UPGMA dendrogram obtained using Nei and Li similarity indexes of the panel of geographically diverse strains using the RAPD profiles generated with a selection of 9 decamers.
Species assignment according to isoenzyme analysis, zymodeme (MON for Montpellier) when available, and country of origin are indicated. ET: Ethiopia; SA: Saudi Arabia; IN: India; KE: Kenya; SD: Sudan; TN: Tunisia.
Figure 3.
Genomic RFLP analysis using different restriction enzymes and 3 RAPD markers unassigned by in silico analysis.
The hybridization patterns were revealed by the different probes, A: LEM536/320/OPAY8, B: LEM496/300/OPAD17 and C: M106/200/OPAY9. Tested isolates correspond to L. major (FMH; L. maj), L. archibaldi (GEBRE; L. arch), L. donovani (HU3; L. don), L. tropica (DBKM; L. tro) and L. infantum (LEM1163; L. inf). EcoRI, PstI, HindIII, XhoI on top of the panel indicate the restriction enzyme used to digest the total Leishmania DNAs.
Table 3.
Selected features characterizing the cloned RAPD markers.
Table 4.
Comparative inter-species analysis of mutations within the RAPD priming sites.