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Table 1.

Patient Characteristics.

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Figure 1.

Mean plasma (+/− standard error) cytokine levels at each time point during radiotherapy (1-hour, 24-hours, 4-weeks) and after radiotherapy (12-weeks).

Levels are grouped into treatment type (ChemoRT vs RT alone). Cytokines in which the variation is different dependent upon treatment groups are marked with a delta (δ). Within each cytokine graph, an asterisk (*) denotes at which time the level of plasma cytokines are significantly different from the baseline level, with corrections for multiple comparisons performed using Dunnett’s method.

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Figure 2.

Venn diagram depicting the relationship between variation of plasma cytokine levels and the variables of time and treatment.

MCP-1, TIMP-1, IP-10, MCP-3 and MIP-1β vary differently across the different timepoints sampled, but are have similar variance between treatment groups (RT alone vs ChemoRT). IL-6, Eotaxin, MDC, MIP-1α and VEGF levels varied across both time and treatment group. TNF-α levels were no different across time points sampled or treatment delivered.

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Figure 3.

Correlation between irradiated PTV volume and the change in plasma cytokine concentration from baseline to 1-hour post irradiation for IL-6 (A), MCP-1 (B), and IP-10 (C).

Linear-regression line (blue) is displayed with 95% confidence intervals (red broken line).

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Figure 4.

Correlation between mean lung dose (MLD) and the change in plasma cytokine concentration from baseline to 1-hour post irradiation for IL-6 (A), IP-10 (B), MCP-1 (C) and MCP-3 (D).

Linear-regression line (blue) is displayed with 95% confidence intervals (red broken line).

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Figure 5.

Box and Whisker plot demonstrating median population change of cytokine levels from baseline for IP-10 and MCP-1 during treatment delivery, with 5th–95th centiles.

Statistically significant differences between patients with severe respiratory toxicities [shaded boxes] and those without [open boxes] are highlighted with associated p - values.

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Figure 6.

Box and Whisker plot demonstrating median population change of cytokine levels from baseline for Eotaxin, IL-6 and TIMP-1 during treatment delivery, with 5th–95th centiles.

Statistically significant differences between patients with severe respiratory toxicities [shaded boxes] and those without [open boxes] are highlighted with associated p - values.

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Figure 6 Expand